Journal: Nature Communications

Article Title: Immunogenicity of a DNA vaccine candidate for COVID-19

doi: 10.1038/s41467-020-16505-0

Figure Lengend Snippet: Hartley guinea pigs were immunized on Day 0 with 100 µg INO-4800 or pVAX-empty vector as described in the methods. a SARS-CoV-2 S protein antigen binding of IgG in serial serum dilutions at day 0 and 14. Data shown represent mean OD450 nm values (mean + SD) for the five guinea pigs. b Serum IgG binding titers (mean ± SD) to SARS-CoV-2 S protein at day 14. Values depicted are mean ± SD. P values determined by Mann–Whitney test.

Article Snippet: Plates were washed four times with washing buffer then incubated with full length (S1 + S2) spike protein containing a C-terminal His tag (Sino Biologics, cat. 40589-V08B1) at 10 µg mL −1 for 1 h at RT.

Techniques: Plasmid Preparation, Binding Assay, MANN-WHITNEY

Journal: Nature Communications

Article Title: Immunogenicity of a DNA vaccine candidate for COVID-19

doi: 10.1038/s41467-020-16505-0

Figure Lengend Snippet: a Soluble ACE2 receptor binds to CoV-2 full-length spike with an EC 50 of 0.025 µg/ml. b Purified serum IgG from BALB/c mice ( n of 5 per group) after second immunization with INO-4800 yields significant competition against ACE2 receptor. Serum IgG samples from the animals were run in triplicate. c IgGs purified from n = 5 mice day 7 post second immunization with INO-4800 show significant competition against ACE2 receptor binding to SARS-CoV-2 S 1 + 2 protein. The soluble ACE2 concentration for the competition assay is ~0.1 µg ml −1 . Bars represent the mean and standard deviation of AUC for curves displayed in Supplementary Fig. . d Hartley guinea pigs were immunized on Day 0 and 14 with 100 µg INO-4800 or pVAX-empty vector as described in the methods. Day 28 collected sera (diluted 1:20) was added SARS-CoV-2 coated wells prior to the addition of serial dilutions of ACE2 protein. Detection of ACE2 binding to SARS-CoV-2 S protein was measured. Sera collected from 5 INO-4800-treated and 3 pVAX-treated animals were used in this experiment. e Serial dilutions of guinea pig sera collected on day 21 were added to SARS-CoV-2 coated wells prior to the addition of ACE2 protein. Detection of ACE2 binding to SARS-CoV-2 S protein was measured. Sera collected from 4 INO-4800-treated and 5 pVAX-treated guinea pigs were used in this experiment.

Article Snippet: Plates were washed four times with washing buffer then incubated with full length (S1 + S2) spike protein containing a C-terminal His tag (Sino Biologics, cat. 40589-V08B1) at 10 µg mL −1 for 1 h at RT.

Techniques: Purification, Binding Assay, Concentration Assay, Competitive Binding Assay, Standard Deviation, Plasmid Preparation

Journal: Journal of Inflammation Research

Article Title: In vivo and in vitro Evaluation of Cytokine Expression Profiles During Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection

doi: 10.2147/JIR.S312337

Figure Lengend Snippet: Demographic Data from the MERS-CoV-Infected Group and Healthy Noninfected Group (Collected in 2019)

Article Snippet: A recombinant version of the full-length MERS-CoV spike protein (Sino Biological) was used at 5 µg/mL to stimulate the cells.

Techniques: Infection

Journal: Journal of Inflammation Research

Article Title: In vivo and in vitro Evaluation of Cytokine Expression Profiles During Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection

doi: 10.2147/JIR.S312337

Figure Lengend Snippet: The level of IFNs in MERS-CoV-infected patients. Serum was assessed for expression of the IFNs IFN-γ ( A ), IFN-ß ( B ) and IFN-α2 ( C ) in two different groups (MERS-CoV-infected patients vs the healthy noninfected group). All data are presented as the median (25 th -75 th percentile), and * P -value < 0.05 indicates statistical significance.

Article Snippet: A recombinant version of the full-length MERS-CoV spike protein (Sino Biological) was used at 5 µg/mL to stimulate the cells.

Techniques: Infection, Expressing

Journal: Journal of Inflammation Research

Article Title: In vivo and in vitro Evaluation of Cytokine Expression Profiles During Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection

doi: 10.2147/JIR.S312337

Figure Lengend Snippet: Differences in the concentrations of IL-8, IL-2, and IL-12 in MERS-CoV patients compared to the healthy noninfected group. Serum was assessed for the expression of IL-8 ( A ), IL-2 ( B ), IL-12p40 ( C ), and IL-12p70 ( D ) in two different groups (MERS-CoV-infected patients vs healthy noninfected group as the control). All data are presented as the median (25 th -75 th percentile), and * P -value < 0.05 indicates statistical significance.

Article Snippet: A recombinant version of the full-length MERS-CoV spike protein (Sino Biological) was used at 5 µg/mL to stimulate the cells.

Techniques: Expressing, Infection

Journal: Journal of Inflammation Research

Article Title: In vivo and in vitro Evaluation of Cytokine Expression Profiles During Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection

doi: 10.2147/JIR.S312337

Figure Lengend Snippet: Levels of the cytokines IL-10, IL-26 and IL-35. Serum was assessed for the expression of IL-10 ( A ), IL-26 ( B ), IL-35 ( C ), and IL-27 ( D ) in two different groups (MERS-CoV-infected patients vs the healthy noninfected group as the control). All data are presented as the median (25 th -75 th percentile), and * P -value < 0.05 indicates statistical significance.

Article Snippet: A recombinant version of the full-length MERS-CoV spike protein (Sino Biological) was used at 5 µg/mL to stimulate the cells.

Techniques: Expressing, Infection

Journal: Frontiers in Immunology

Article Title: Effect of seasonal coronavirus immune imprinting on the immunogenicity of inactivated COVID-19 vaccination

doi: 10.3389/fimmu.2023.1195533

Figure Lengend Snippet: Seroprevalence of sCoVs and SARS-CoV-2 S IgG antibodies in different cohorts.

Article Snippet: Briefly, to detect corresponding antibodies, 96-well EIA/RIA plates (Corning, #3590) were coated with recombinant SARS-CoV-2 full-length S (Sino Biological, #40591-V08B1-1), SARS-CoV-2 S1(Sino Biological, #40591-V08H), S2 (Sino Biological, #40590-V08B), SARS-CoV-2 full-length N (Sino Biological, #40588-V08B), HCoV-HKU1 full-length S (Sino Biological, #40606-V08B), HCoV-NL63 full-length S (Sino Biological, #40604-V08B), HCoV-OC43 full-length S (Sino Biological, #40607-V08B), and HCoV-229E full-length S proteins (Sino Biological, #40605-V08B) at 100ng/ul in ELISA coating buffer (Solarbio, #C1055) respectively, and incubated at 4°C overnight.

Techniques: